MCB 5427-50 Laboratory Techniques in Functional Genomics
Topic – Introduction to Cell Culture Techniques
Dates: May 16, May 18, May 20, 2022, 9:00 AM - 5:00 PM
1 credit
In Person
Enrollment Limit 6
Instructor: Sau-Mei Leung
Coordinator: Charles Giardina
Room TLS 253
Instructor Consent Required

Contact elaine.mirkin@uconn.edu for permission number. Prerequisite is MCB 5427 Introduction to Molecular Biology Techniques, relevant research lab experience, or permission of instructor. Participants learn the key concepts, problems, and methods for obtaining, growing, storing, and using animal cells. Main topics include media preparation and filtration, aseptic techniques, basic phase contrast microscopy, setting up cultures from frozen vials, the use of Biological Safety Cabinet, monolayer culture techniques, subcultivation, cell counting, cell freezing techniques, dilution plating, managing culture contamination, toxicity testing using 96 well plates, mycoplasma testing, and monolayer transfection.

MCB 5671-03 Advanced Theory and Practice of Laboratory Techniques in Microbiology
Topic – COVID-19 Diagnosis: SARS-CoV-2 Quantification, Mutation Detection and Genome Sequencing
Dates: May 9-13, 2022: 9:00 AM - 5:00 PM
2 credits
In Person
Enrollment limit 6
Instructors:  Todd Testerman, and Angeline Casale
Coordinator: Joerg Graf
Rooms BCH 204
May 9-13, 2022: 9:00 AM - 5:00 PM
Instructor Consent Required

Contact elaine.mirkin@uconn.edu for permission number. Prerequisite is MCB 5427 or permission from the instructor. In this module, participants use qPCR to quantify SARS-CoV-2 and detect the presence of mutations that occur in variants. In addition, the Oxford Nanopore’s minION and Mk1C will be used to sequence genomes of SARS-CoV-2 and confirm the presence of mutations in the viral genome.