James Cole

Professor Emeritus

Molecular and Cell Biology / Structural Biology, Biochemistry and Biophysics (SB3)

Retired: 2023

Education: Ph.D., University of California; Post-doctoral study, Stanford University.

Research Interests: We use biochemical, biophysical and structural methods to define the key macromolecular interactions that regulate important biological processes. Our major focus is the innate immunity pathway for defense against viral infection.

Selected Publications:

Erlandsen, H., Jecrois, A.M., Nichols, J.C., Cole, J.L. and Royer, W.E., Jr (2022), NADH/NAD+ binding and linked tetrameric assembly of the oncogenic transcription factors CtBP1 and CtBP2. FEBS Letters. Accepted Author Manuscript. https://doi.org/10.1002/1873-3468.14276

Stephen Hesler, Matthew Angeliadis, Bushra Husain, and James L. Cole. 2021. Contribution of dsRBD2 to PKR Activation. ACS Omega DOI: 10.1021/acsomega.1c00343

Sujit Basak, Nabanita Sakia, Laura Dougherty, Zhuojun Guo, Fang Wu, Frank Mindlin, Jeffrey W. Lary, James L. Cole, Feng Ding, Mark E. Bowen, Probing Interdomain Linkers and Protein Supertertiary Structure In Vitro and in Live Cells with Fluorescent Protein Resonance Energy Transfer, Journal of Molecular Biology, Volume 433, Issue 5, 2021, 166793, ISSN 0022-2836, https://doi.org/10.1016/j.jmb.2020.166793.

Zerbe, Cassie M, and Cole, James L. “Regulation of PKR by Epstein-Barr Virus EBER1 RNA.” Biochemistry.Ja (2020): Biochemistry. , 2020, (ja). Web.

Zerbe, C.M., Mouser, D.J. and Cole, J.L. (2019) Oligomerization of RIG-I and MDA5 2CARD Domains. Protein Science, in press.

Mayo, C.B., Erlandsen, H., Mouser, D.J., Feinstein, A.G., Robinson, V.L., May, E.R., Cole, J.L. (2019) Structural Basis of Protein Kinase R Autophosphorylation. Biochemistry 58, 2967-2977.

Cole, J.L. (2018) Catalytic Domains in RNA-Binding Proteins. In Roberts G., Watts A., European Biophysical Societies (eds.) Encyclopedia of Biophysics. Springer, Berlin, Heidelberg.

Pindrus, M.A., Cole, J.L., Kaur, J., Shire, S.J., Yadav, S. and Kalonia, D.S. (2017) Effect of aggregation on the hydrodynamic properties of bovine serum albumin. Pharm. Res.34, 2250-2259.

Mayo, C.B. and Cole, J.L. (2017) Interaction of PKR with single-stranded RNA. Sci. Rep.7, 3335.

Mayo, C.B., Wong, C.J., Lopez P.E., Lary, J.W., Cole, J.L. (2016) Activation of PKR by short stem-loop RNAs containing single-stranded arms. RNA 22, 1065-175.

Husain, B., Mayo, C. and Cole, J.L. (2015) Role of the interdomain linker in PKR activation. Biochemistry 55, 253-261.

Husain, B., Hesler, S. and Cole, J.L. (2015) Regulation of PKR by RNA: Formation of active and inactive dimers. Biochemistry 54, 6663-6672.

Launer-Felty, K., Wong, C.J. and Cole, J.L. (2014) Structural analysis of Adenovirus VAI RNA defines the mechanism of inhibition of PKR. Biophysical Journal 108, 748-757.

Kaus, K., Lary, J.W., Cole, J.L. and Olson, R. (2014) Glycan specificity of the Vibrio vulnificus hemolysin lectin outlines evolutionary history of membrane targeting by a toxin family. J. Mol. Biol. 426, 1285-1295.

Wowor, A.J., Yan, Y., Auclair, S.M., Yu, D., Zhang, J., May, E.R., Gross, M.L., Kendall, D.A. and Cole, J.L. (2014) Analysis of SecA Dimerization in Solution. Biochemistry 53, 3248-3260.

Launer-Felty, K. and Cole, J.L. (2014) Domain interactions in Adenovirus VAI mediate high-affinity PKR binding. J. Mol. Biol. 426, 1285-1295.

Jim Cole
Contact Information
Mailing Address91 North Eagleville Road, Unit 3125, Storrs, CT 06269-3125
Office LocationBiology/Physics Building 205