James Cole


University of Connecticut
Department of Molecular & Cell Biology
91 North Eagleville Road, Unit-3125
Biology/Physics Building 205
Storrs, CT 06269-3125

Telephone: (860) 486-4333
Fax: (860) 486-4331

Visit Professor┬áCole’s Website.

Education: Ph.D., University of California; Post-doctoral study, Stanford University.

Research Interests: Characterization of macromolecular interactions using analytical ultracentrifugation and related biophysical techniques. Double-stranded RNA activated protein kinase (PKR); interferon antiviral defense pathway; trp RNA-binding attenuation Protein (TRAP).

Selected Publications:

Pindrus, M.A., Cole, J.L., Kaur, J., Shire, S.J., Yadav, S. and Kalonia, D.S. (2017) Effect of aggregation on the hydrodynamic properties of bovine serum albumin. Pharm. Res. DOI:10.1007/s11095-017-2231-2

Mayo, C.B. and Cole, J.L. (2017) Interaction of PKR with single-stranded RNA. Sci. Rep.7, 3335

Mayo, C.B., Wong, C.J., Lopez P.E., Lary, J.W., Cole, J.L. (2016) Activation of PKR by short stem-loop RNAs containing single-stranded arms. RNA, DOI:10.1261/rna.053348.115.

Husain, B., Mayo, C. and Cole, J.L. (2015) Role of the interdomain linker in PKR activation. Biochemistry, in press, DOI:10.1021/acs.biochem.5b01171.

Husain, B., Hesler, S. and Cole, J.L. (2015) Regulation of PKR by RNA: Formation of active and inactive dimers. Biochemistry 54, 6663-6672.

Launer-Felty, K., Wong, C.J. and Cole, J.L. (2014) Structural analysis of Adenovirus VAI RNA defines the mechanism of inhibition of PKR. Biophysical Journal 108, 748-757.

Kaus, K., Lary, J.W., Cole, J.L. and Olson, R. (2014) Glycan specificity of the Vibrio vulnificus hemolysin lectin outlines evolutionary history of membrane targeting by a toxin family. J. Mol. Biol. 426, 1285-1295

Wowor, A.J., Yan, Y., Auclair, S.M., Yu, D., Zhang, J., May, E.R., Gross, M.L., Kendall, D.A. and Cole, J.L. (2014) Analysis of SecA Dimerization in Solution. Biochemistry 53, 3248-3260.

Launer-Felty, K. and Cole, J.L. (2014) Domain interactions in Adenovirus VAI mediate high-affinity PKR binding.J. Mol. Biol. 426, 1285-1295.

Yu, D., Wowor, A., Cole, J.L., and Kendall, D.A. (2013). Defining the Escherichia coli SecA Dimer Interface Residues through in Vivo Site-specific Photo-crosslinking.J. Bacteriol. 195, 2817-2825.

Ehlinger, A., Park, S., Fahmy, A., Lary, J.W., Cole, J.L., Finely, D., and Walters, K.J. (2013). Conformational Dynamics of the Rpt6 ATPase in Proteasome Assembly and Rpn14 Binding.Structure 21, 735-765

Cole, J.L, Correia, J.J. and Stafford, W.F. (2011) The use of analytical sedimentation velocity to extract thermodynamic linkage Biophys. Chem. 159, 120-128.

Wong, C.J., Launer-Felty, K. and Cole, J.L. (2011) Analysis of PKR-RNA interactions by sedimentation velocity. Methods Enzymol. 488, 59-79.