University of Connecticut
Department of Molecular & Cell Biology
91 North Eagleville Road, Unit 3125
Biology/Physics Building 206
Storrs, CT 06269-3125
Telephone: 860- 486-4282
Education: Ph.D., Washington State University; Post-doctoral training, Massachusetts Institute of Technology
Research Interests: Biochemical, biophysical, and mutational analysis of virus assembly, especially of the thermodynamics of capsid assembly and maturation reactions.
-The role of the two SecA homologs in protein export in Mycobacterium tuberculosis.
-Protein folding in vivo and in vitro. Interaction of folding intermediates with molecular chaperones.
Asija K, Teschke CM. 2018. Lessons from bacteriophages part 1: Deriving utility from protein structure, function, and evolution. PLoS Pathog. DOI: 10.1371/journal.ppat.1006971
Asija K, Teschke CM. 2018. Lessons from bacteriophages part 2: A saga of scientific breakthroughs and prospects for their use in human health. PLoS Pathog. DOI: 10.1371/journal.ppat.1006970
Tina Motwani, Ravi K. Lokareddy, Carmen A. Dunbar, Juliana R. Cortines, Martin F. Jarrold, Gino Cingolani, Carolyn M. Teschke (2017) A viral scaffolding protein triggers portal ring oligomerization and incorporation during procapsid assembly. Science Advances DOI: 10.1126/sciadv.1700423.
Suhanovsky, M., Teschke, C. (2015) Nature׳s favorite building block: Deciphering folding and capsid assembly of proteins with the HK97-fold. Virology doi:10.1016/j.virol.2015.02.055.
Rizzo, A.A., Suhanovsky, M.M., Baker, M.L., Fraser, L.C.R., Jones, L.M., Rempel, D.L., Gross, M.L., Chiu, W., Alexandrescu, A.T. & Teschke, C.M. (2014) “Multiple functional roles of the accessory I-domain of bacteriophage P22 coat protein revealed by NMR structure and cryoEM modeling”. Structure 22, 830-841.
Cortines JR, Motwani T, Vyas AA, Teschke CMJ Virol. (2014) Highly specific salt bridges govern bacteriophage P22 icosahedral capsid assembly: identification of the site in coat protein responsible for interaction with scaffolding protein. May;88(10):5287-97. doi: 10.1128/JVI.00036-14. Epub 2014 Mar 5.
Padilla-Meier GP, Gilcrease EB, Weigele PR, Cortines JR, Siegel M, Leavitt JC, Teschke CM, Casjens SR (2012) Unraveling the Role of the C-terminal Helix-Turn-Helix of the Coat-binding Domain of Bacteriophage P22 Scaffolding Protein. J Biol Chem. 2012 Aug 9. [Epub ahead of print]
Teschke CM. (2012) Themes and variations of viral small terminase proteins. Structure 20(8): 1291-2.
Parent KN, Deedas CT, Egelman EH, Casjens SR, Baker TS, Teschke CM (2012) Stepwise molecular display utilizing icosahedral and helical complexes of phage coat and decoration proteins in the development of robust nanoscale display vehicles. Biomaterials 33(22):5628-37. Epub 2012 May 8.
Zlotnick A, Suhanovsky MM, Teschke CM (2012) The energetic contributions of scaffolding and coat proteins to the assembly of bacteriophage procapsids. Virology 428(1):64-9. Epub 2012 Apr 20
Cortines JR, Weigele PR, Gilcrease EB, Casjens SR, Teschke CM (2011) Decoding bacteriophage P22 assembly: identification of two charged residues in scaffolding protein responsible for coat protein interaction. Virology 421(1):1-11. Epub 2011 Oct 4
Suhanovsky MM, Teschke CM (2011) Bacteriophage P22 capsid size determination: roles for the coat protein telokin-like domain and the scaffolding protein amino-terminus.
Virology 417(2):418-29. Epub 2011 Jul 23.
Padilla-Meier GP, Teschke CM (2011) Conformational changes in bacteriophage P22 scaffolding protein induced by interaction with coat protein. J Mol Biol 410(2):226-40. Epub 2011 May 14.
Parent KN, Sinkovits RS, Suhanovsky MM, Teschke CM, Egelman EH, Baker TS. (2010) Cryo-reconstructions of P22 polyheads suggest that phage assembly is nucleated by trimeric interactions among coat proteins.Phys Biol. 7(4):045004
Suhanovsky MM, Parent KN, Dunn SE, Baker TS, Teschke CM (2010) Determinants of bacteriophage P22 polyhead formation: the role of coat protein flexibility in conformational switching.Mol Microbiol 77(6):1568-82 Epub 2010 Aug 18
Teschke CM, Parent KN (2010) Let the phage do the work: using the phage P22 coat protein structures as a framework to understand its folding and assembly mutants. Virology 401(2):119-30. Epub 2010 Mar 16.
Parent KN, Khayat R, Tu LH, Suhanovsky MM, Cortines JR, Teschke CM, Johnson JE, Baker TS (2010) P22 coat protein structures reveal a novel mechanism for capsid maturation: stability without auxiliary proteins or chemical crosslinks. Structure 18(3):390-401
Parent KN, Khayat R, Tu LH, Suhanovsky MM, Cortines JR, Teschke CM, Johnson JE, Baker TS. P22 coat protein structures reveal a novel mechanism for capsid maturation: stability without auxiliary proteins or chemical crosslinks. Structure. 2010 Mar 10;18(3):390-401
Hou, J.M., D’Lima, N.G., Rigel, R.W., Gibbons, H.S., Braunstein, M., Teschke, C.M. (2008) ATPase activity of Mycobacterium tuberculosis SecA1 and SecA2 proteins and its importance to SecA2 function in macrophages. J. Bacteriol., 190, 4880-7.
Parent, K.N., Suhanovsky, M.M., Teschke, C.M. (2007) Polyhead formation in phage P22 pinpoints a region in coat protein required for conformational switching. Molecular Microbiol. 65,1300-10.
Parent, K.N., Teschke, C.M. (2007) GroEL/S substrate specificity based on substrate unfolding propensity. Cell Stress and Chaperones, 12, 20-32.
Parent, K.N., Suhanovsky, M.M., Teschke, C.M. (2007) Phage P22 procapsids equilibrate with free coat protein subunits. J. Mol. Biol., 365, 513-22.
Parent 4. K.N., Zlotnick, A., Teschke, C.M. (2006) Quantitative analysis of multi-component spherical virus assembly: Scaffolding protein contributes to the global stability of phage P22 procapsids. J. Mol. Biol., 359,1097-106.