University of Connecticut
Department of Molecular & Cell Biology
91 North Eagleville Road, Unit 3125
Biology/Physics Building 306
Storrs, CT 06269-3125
Education: Ph.D. 1986, University of London
Research Interests: Cell movement is a highly complex phenomenon which is itself composed of several other “motile processes”, such as protrusion, adhesion, contraction and detachment. The overall aim of my work is to understand how molecular mechanisms and biomechanical properties are integrated at the cellular level to produce movement. This requires learning how the dynamic behavior of the actin cytoskeleton and cell – substratum adhesion formation is regulated both spatially and temporally. I am particularly interested in the mechano – chemical regulation of cell movement as this is important for understanding the interrelationship between molecular processes, force production, cell morphology and movement.
So far my studies have focused primarily on fish epithelial keratocytes because their rapid, relatively simple mode of movement is best suited for discerning the basic principles which relate molecular events to whole cell movement. I use a combination of techniques including fluorescence video microscopy, calcium imaging, photoactivation, and force detection assays to observe molecular, cellular and biophysical aspects of cell movement.
Lee, J. Ishihara, A., Theriot, J.A. and Jacobson, K. (1993). Principles of locomotion for simple-shaped cells. Nature 362, 167-171.
Lee, J., Leonard, M., Oliver, T., Ishihara, A. and Jacobson, K. (1994). Traction forces generated by locomoting keratocytes. J Cell Biol. 127, 1957-1964
Lee, J. and Jacobson, K. (1997). The composition and dynamics of cell -substratum adhesions in locomoting fish keratocytes. (1997). J. Cell Sci. 110, 2833-2844.
Lee, J., Ishihara, A., Oxford, G. Johnson, B. and Jacobson, K. (1999). The regulation of cell movement is mediated by stretch – activated calcium channels Nature, 400, 382-386.