Professor Dan Gage Among UConn Researchers Involved in Study to use Mussels in the Filtration of Microplastics

March 15, 2021

Professor Dan Gage among UConn researchers involved in a study to use mussels in the filtration of microplastics funded by a $2 million grant from the National Science Foundation's Emerging Frontiers in Research and Innovation (EFRI). They will study the use of mussels (part of the bivalve family), combined with microplastic-degrading bacteria, in the filtration of microplastics from the discharge that flows back into our surface water from wastewater treatment plants.

How Marine Animals Could Be Used to Clean Up Nature’s Big Pollutant: Microplastics

'Nature's perfect filtering machines' to the rescue

n a hot summer day in Connecticut, it’s common to go to a beach-side restaurant, eat some fresh oysters and mussels, and enjoy the crashing of the waves against the sand. For a group of University of Connecticut faculty and a Florida Atlantic University professor, their plan is to skip the beach and the restaurant and use relatives of those delicious animals for another reason—filtering the harmful microplastics that end up back in our environment.

“Suspension-feeding bivalves, such as oysters, clams, and zebra mussels are very efficient at filtering water and capturing on their gills (the ‘filter’) particles as small as four micrometers in size [less than 1000th of an inch]. Their ‘filter’ is self-cleaning and they often filter water for 12 or more hours per day.  They are nature’s perfect filtering ‘machine,’” Marine Sciences Professor J. Evan Ward says.

Over the next four years, the group – including Associate Dean Leslie Shor, Chemical and Biomolecular Engineering Professor Kelly Burke, Molecular and Cell Biology Professor Daniel Gage, Civil and Environmental Engineering Professor Baikun Li, and Ward – will use a $2 million grant from the National Science Foundation’s Emerging Frontiers in Research and Innovation (EFRI) program to study the use of mussels (part of the bivalve family), combined with microplastic-degrading bacteria, in the filtration of microplastics from the discharge that flows back into our surface water from wastewater treatment plants.

Other faculty members involved in the project include CEE Professor Christine Kirchhoff, CBE Professor Matthew Stuber, CBE Professor Jeff McCutcheon, Marine Sciences Professor George McManus, and Florida Atlantic University Biology Professor Tracy Mincer.

Microplastics, an umbrella term for particles of many different shapes, sizes (<5 mm), and polymer types, are commonly found in the environment through the shedding of synthetic fibers that wash off clothes in the laundry and tiny plastic fragments that are produced in the environment by different processes.

“Most wastewater treatment plants rely on old technology–over 100 years-old–and in some cases use basic approaches like sand filtration that have been known since ancient times,” Li says. “In fact, most wastewater treatment plants around the nation are themselves over 50-years old. When these facilities were designed and built, plastics simply did not exist in the variety or quantity that they do today.”

Kirchhoff explains that even if the technical hurdles are overcome, there still may be a problem.

“Retrofitting existing infrastructure is an expensive proposition, and there are also many regulatory obstacles standing in the way. Better understanding the non-science obstacles to implementing innovative technology is a key aspect of our research project.”

The Water Pollution Control Facility at UConn.
UConn’s Water Pollution Control Facility (courtesy of Baikun Li).

Because of the limitations of wastewater treatment, and also because larger plastics break down in the environment, microplastics end up all over our environment, and many types are tough to break down. The concern is that microplastics could cause harm to animals, plant life, and eventually humans.

According to Mincer, it has been shown that plastic particles less than 150 micrometers can make their way into our lymphatic systems, causing systemic exposure and, perhaps, affecting human health.

“Microplastics can also act as sponges, gathering up other harmful things in the environment. Many studies have shown that concentrations of other common contaminants such as harmful chemicals, pathogenic bacteria, and even viruses can be much higher in microplastics than they are in the surrounding water. Consuming microplastics is therefore a way to be exposed to other harmful contaminants,” Mincer says.

In the end, the group hopes learning from nature and working with stakeholders on the barriers to adopting new technology will lead to a sustainable way to better treat wastewater.

“If the project is successful, not only will we develop innovative microplastic wastewater treatment technology, but we will also quantify drivers and barriers to adoption of this new technology with the ultimate goal of increasing its uptake,” Kirchhoff says.

The group also received a Research Experience & Mentoring supplement for their award for the summer of 2021, and in addition to recruiting graduate students, are currently recruiting undergraduates, high school students, and local teachers for paid summer projects. For more information on Shor’s research, please click here.

Collaboration Between Researchers at UConn, CCMC, and Shoreline Biome Yields Promising Findings about Bacterial Infection in Premature Infants in the NICU.

March 2, 2021

UConn Researchers Collaborate with TIP Company Shoreline Biome to Study Microbiome in NICU

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Bacteria tend to get a bad rap. But oftentimes it’s one bad apple that ruins the reputation of the bunch.

For example, E. coli, commonly known as bacteria that cause sickness, also has beneficial strains that can help protect us from pathogens. Being able to differentiate between bacterial strains is critical for researchers working to understand the microbiome – the complex environment of bacteria living in and on our bodies.

A collaboration between researchers at UConn, Connecticut Children’s, and Technology Incubation Program company Shoreline Biome has yielded promising findings about bacterial infection in premature infants in the Neonatal Intensive Care Unit (NICU). The group published their findings in mBio in February.

Using Shoreline Biome’s patented microbiome assay technology, the group was able to identify previously un-sequenced bacterial strains that appeared in the stool microbiome of two sets of twins in the Connecticut Children’s NICU.

“It gives us unprecedented resolution and the ability to differentiate bacteria to the species or even strain level,” Joerg Graf, UConn professor of molecular and cell biology, says.

Premature infants in the NICU are at a high risk of infections because their immune system and digestive tract are not fully developed. The researchers were particularly interested in intestinal infections caused by certain strains of Klebsiella. They also identified strains of Escherichia coli, and Enterobacter.

The researchers identified unique microbiome fingerprints between and within the sets of twins. This provided valuable insight into the colonization processes in the NICU by looking at which bacteria appear and how they spread.

Normally, the first two years of life, starting the moment a baby goes through the birth canal, is a critical period for developing a healthy microbiome to carry for the entire lifetime. Babies in the NICU are rigorously protected from possible infection, which helps prevent them from getting sick, but also disrupts normal colonization patterns of commensal bacteria.

The researchers plan to continue this research in NICUs in other geographic areas, other parts of the hospital, and other microbiomes, such as those on our skin or in our mouths.

The researchers hope to eventually be able to detect a single genetic fingerprint associated with infection which would pave the way for better strategies to track and combat harmful bacteria.

“That would be a critical finding to improving the overall health of premature babies,” Adam Matson, Connecticut Children’s researcher and assistant professor of pediatrics and immunology at UConn Health, says.

Shoreline Biome’s high-throughput, high-resolution technology sequences a large portion of the ribosomal operon. This part of the bacterial genome contains highly conserved, or similar regions, and other regions that are very diverse. The conserved regions make it possible to use polymerase chain reaction amplification to make thousands of copies of the bacteria’s genetic information.

After sequencing, researchers can study other variable regions of the operon to identify the bacteria more precisely. This also helps identify a bacterium’s evolutionary relationship to known bacteria.

There are two ways to open bacteria to look at their genomes: cracking them open hard, which risks damaging the DNA, or doing it more gently and risking not accessing the DNA from difficult to crack bacteria. Shoreline Biome’s technology combines the best of both, cracking open all bacteria without damaging the DNA.

“If you don’t see DNA, you can’t sequence it,” Mark Driscoll, Shoreline Biome co-founder and chief scientific officer, says. “Now that we can see it, we can actually start to study it.”

Shoreline Biome’s technology allows researchers to analyze multiple samples at once. This is a major advantage for microbiome research. Many microbiome studies suffer from having small sample sizes due to the technical difficulty and costs of analyzing the samples.

Shoreline Biome’s technology delivers scientists and physicians with actionable results. Knowing which strains are showing up in patient biomes can help them to track where they are coming from.

Eventually, this could lead to rapid tests to see if someone entering the NICU is carrying a harmful bacterium. Conversely, they could also track beneficial bacteria infants should be exposed to.

“We’re actively looking at ways to apply this technology to track pathogens and encourage colonization of healthy microbiomes,” Matson says.

The collaboration between Shoreline Biome and the researchers extends beyond this project. Graduate students from the Professional Science Master’s Programs in Applied Microbial Systems Analysis Graf directs now work for Shoreline Biome, retaining a valuable workforce in the state.

“It’s great for us to be able to work with experts to deepen the technology,” Driscoll says. “It’s a great ecosystem.”

 

2021 MCB Grad Recruitment a Virtual Success!

February 18, 2021

MCB hosted over 30 grad recruits on Friday, February 5th. The first all-virtual recruitment event was a success and included virtual lab tours, grad student turbo talks, interviews, poster sessions, research seminars and a social gathering to close the day's events. See images!

Professors Lynes and Graf part of a team that received a multi-institution grant to study syndrome affecting children with Covid 19

January 5, 2021

Michael Lynes and Joerg Graf are part of a multi-institution grant to develop biomarker signatures for MIS-C (multisystem inflammatory syndrome in children) that can develop children infected with coronavirus. The Lynes research team will use the grating coupled surface plasmon resonance imaging systems that they have developed with Ciencia, Inc. to measure biomarker signatures in serum and saliva, and the Graf group will identify the composition of the microbiome in the saliva of these patients.  In concert with measures made at the NY Department of Health, Connecticut Children’s Hospital, Jackson Laboratory of Genomic Medicine, and NYU, the plan is to use these biomarker signatures both to diagnose and predict the course of MIS-C disease, and to suggest new and effective therapeutic interventions.

Learn more about the NIH studies

MCB PhD Candidate Kate Castellano Receives 2020 GIGA Fellowship

December 21, 2020

The Global Invertebrate Genomics Alliance (GIGA) has announced that Kate Castellano has received the inaugural GIGA Fellowship in Invertebrate Genomics 2020 Award.

KateCastellano_small

GIGA is dedicated to promoting resources and standards that will facilitate comparative approaches and collaborations for future generations.

About Kate GIGA stated, “With these objectives in mind, we are excited to see Kate’s research that investigates reproductive life history of salps, a group that is in need for expanded genomic resources. We were impressed by her research statement, thoughtful budget and her desire to train future generations in invertebrate –OMICS research.”

Learn more about GIGA

Dr. Kat Milligan-Myhre featured in Indigenous at UConn Video

December 2, 2020

MCB Assistant Professor, Kat Milligan-Myhre was featured in a recent video, Indigenous at UConn, created by UConn Native American Cultural Programs. In the video,  Milligan-Mhyre speaks about both her culture and her science.

“Honk if you Love Squid” MCB’s Sarah McAnulty and her Squidmobile Get Noticed

November 30, 2020

Assistant Research Professor, Sarah McAnulty, was recently featured in an article, "When you text the Squidmobile, this Fishtown scientist texts back squid words of wisdom" in the Philadelphia Inquirer. When squid biologist Sarah McAnulty decided to cover her Toyota RAV4 with paintings of squid and write the words “WANT A SQUID FACT? Text 9-RUNG-SQUID” on the back, she was ready for things to get weird. And she was not disappointed. Read full article

Squidmobile

Alder Research Group Receives Multiple Grant Awards

October 28, 2020

The Alder Lab, Nathan Alder PI, was recently awarded three substantial grants. The first, a grant from the National Institute on Aging, (R01AG065879), "First-in-class Peptide Therapeutics for Mitochondrial Disorders: Molecular Mechanism of Action and Optimization of Design." This five-year R01 for $2.5M is focused on investigating the molecular basis by which mitochondria-targeted peptide compounds interact with membranes and their downstream effects on membrane biophysical properties, protein complex structure and function, and mitochondrial physiology. This is a highly multidisciplinary project that includes collaborators within MCB and from The Johns Hopkins University School of Medicine, and Alexandria LaunchLabs. View Abstract   
Alder's research was featured in UConn Today.

The Barth Syndrome Foundation awarded the group a $50K grant to develop peptide-based therapeutics specifically for the treatment of Barth Syndrome (BTHS), "Development of Mitochondria-Targeted Peptide Compounds as Barth Syndrome Therapeutics." BTHS is an X-linked genetic disease resulting from defects in the transacylase enzyme tafazzin, involved in biosynthetic remodeling of the mitochondrial phospholipid cardiolipin. Using a host of biophysical approaches with model membrane systems and disease models, this work will explore a library of compound variants optimized as therapeutics for treating dysfunctional cardiolipin biogenesis. Learn more about how this grant will be used.

The group also received a grant from the National Institute of General Medical Sciences Grant (R01GM136975), "Mitochondrial Membrane Compartmentalization". This is a multi-PI grant, with collaborator Dr. Steven Claypool (JHU School of Medicine), for $774K over two years. The objective of this work is to elucidate spatial and temporal distribution of lipids and proteins within the subcompartments of the morphologically complex mitochondrion. This will identify how the organelle establishes its ultrastructure as well as differences in spatiotemporal macromolecular distribution relevant to human disease and cellular stressors. This work utilizes novel membrane-active copolymers that extract membrane nanoparticles amenable for protein and lipid analysis. View Abstract

To learn more about the Alder Lab and their research, visit their lab website.

MCB Successfully Finds New Classroom Solutions to Keep Students Safe During the Pandemic.

October 22, 2020

Professors, graduate assistants and support staff have made significant adjustments to courses, preparing multiple lesson plans to meet the needs of in-person and distance learners.

MCB 2610
Molecular and cell biology graduate student Ashley Reed leads a virtual session of MCB 2610: Fundamentals of Microbiology in the Torrey Life Sciences Building on October 6, 2020. (Bri Diaz/UConn Photo)

Andong Li, a graduate student the Department of Molecular and Cell Biology, leads an online lab section of MCB 2000 on October 7, 2020. (Bri Diaz/UConn Photo)

Professors, graduate assistants and support staff have made significant adjustments to courses, preparing multiple lesson plans to meet the needs of in-person and distance learners.
Molecular and cell biology graduate student Joshua Calabrese leads a lab section of MCB 3010: Biochemistry in the Torrey Life Sciences Building on October 6, 2020. (Bri Diaz/UConn Photo)

Story in UConn Today