Kenneth Campellone

Campellone
Assistant Professor

University of Connecticut
Departmentof Molecular and Cell Biology
354 Mansfield Road, Unit 1131
Beach Hall 246
Storrs, CT 06269 -1131

Telephone: 860-486-3326 (office) 860-486-2497 (lab)
Fax: 860-486-1936
kenneth.campellone@uconn.edu

Visit Assistant Professor Campellone’s Website.

 

Education: Postdoc, Molecular and Cell Biology, UC Berkeley; Ph.D., Molecular Genetics and Microbiology, UMass Medical School; B.S., Biology, Providence College

Research Interests: My research goals are to determine (1) how the actin and microtubule cytoskeletons control the organization, shape, and movement of membranes in mammalian cells and (2) how cytoskeleton-driven membrane remodeling is altered during the pathogenesis of inherited and infectious diseases.  A combination of genetic, biochemical, and cellular approaches are used to study these processes.

Selected Publications:

Research Papers
Russo AJ, Mathiowetz AJ, Hong S, Welch MD, and Campellone KG (2016).  Rab1 recruits the actin nucleation machinery but limits filament assembly during membrane remodeling.  Mol Biol Cell. 27(6):967-978. (Cover)

Shen QT, Hsiue PP, Sindelar CV, Welch MD, Campellone KG, and Wang HW (2012). Structural insights into WHAMM-mediated cytoskeletal coordination during membrane remodeling. J Cell Biol. 199:111-124.

Campellone KG, Siripala AD, Leong JM, and Welch MD (2012). Membrane-deforming proteins play distinct roles in actin pedestal biogenesis by enterohemorrhagic Escherichia coli. J Biol Chem 287: 20613-20624

Huang J, Birmingham CL, Shahnazari S, Shiu J, Zheng YT, Smith AC, Campellone KG, Heo WD, Gruenheid S, Meyer T, Welch MD, Ktistakis NT, Kim PK, Klionsky DJ, and Brumell JH. (2011) Antibacterial autophagy occurs at PI(3)P-enriched domains of the endoplasmic reticulum and requires the Rab1 GTPase. Autophagy. 7(1):17-26.

Vingadassalom D*, Campellone KG*, Brady MJ, Skehan B, Battle SE, Robbins D, Kapoor A, Hecht G, Snapper SB, and Leong JM (2010). Enterohemorrhagic E. coli requires N-WASP for efficient type III translocation but not for EspFU-mediated actin pedestal formation. PLoS Pathog. 6:e1001056 Epub.

Campellone KG, Cheng HC, Robbins D, Siripala AD, McGhie EJ, Hayward RD, Welch MD, Rosen MK, Koronakis V, and Leong JM (2008). Repetitive N-WASP-binding elements of the enterohemorrhagic effector EspFU synergistically activate actin assembly. PLoS Pathog. 4:e1000191 Epub.

Cheng HC, Skehan BM, Campellone KG, Leong JM, and Rosen MK (2008). Structural mechanism of WASP activation by the enterohaemorrhagic E. coli effector EspFU. Nature. 454(7207):1009-1013.

Campellone KG, Webb NJ, Znameroski EA, and Welch MD (2008). WHAMM is an Arp2/3 complex activator that binds microtubules and functions in ER to Golgi transport. Cell. 134(1):148-161.

Campellone KG, Roe AJ, Lobner-Olesen A, Murphy KC, Magoun L, Brady MJ, Donohue-Rolfe A, Tzipori S, Gally DL, Leong JM, and Marinus MG (2007). Increased adherence and actin pedestal formation by dam-deficient enterohaemorrhagic Escherichia coli O157:H7. Mol Microbiol. 63(5):1468-1481.

Campellone KG, Brady MJ, Alamares JG, Rowe DC, Skehan B, Tipper DJ, and Leong JM (2006). Enterohemorrhagic Escherichia coli Tir requires a C-terminal 12-residue peptide to initiate EspFU-mediated actin assembly and harbors N-terminal sequences that influence pedestal length. Cell Microbiol. 8(9):1488-1503.

Campellone KG and Leong JM (2005). Nck-independent actin assembly is mediated by two phosphorylated tyrosines within enteropathogenic Escherichia coli Tir. Mol Microbiol. 56(2): 416-432.

Campellone KG, Robbins D, and Leong JM (2004). EspFU is a translocated EHEC effector that interacts with Tir and N-WASP and promotes Nck-independent actin assembly. Dev Cell. 7(2): 217-228.

Campellone KG, Rankin S, Pawson T, Kirschner MW, Tipper DJ, and Leong JM (2004). Clustering of Nck by a 12-residue Tir phosphopeptide is sufficient to trigger localized actin assembly. J Cell Biol. 164(3): 407-416.

Review Articles
Rottner K, Hanisch J, and Campellone KG (2010). WASH, WHAMM, and JMY: Regulation of Arp2/3 complex and beyond. Trends Cell Biol. 20(11): 650-661.

Campellone KG (2010). Cytoskeleton-modulating effectors of enteropathogenic and enterohaemorrhagic E. coli: Tir, EspFU, and actin pedestal assembly. FEBS J. 277(11): 2390-402.

Campellone KG and Welch MD. (2010). A nucleator arms race: Cellular control of actin assembly. Nat Rev Mol Cell Biol. 11(4): 237-251.

Campellone KG (2010). Phosphoinositides influence pathogen surfing: EPEC rights the SHIP. Cell Host Microbe 7(1):1-2.

Hayward RD, Leong JM, Koronakis V and Campellone KG (2006). Exploiting pathogenic Escherichia coli to model transmembrane receptor signaling. Nat Rev Microbiol. 4(5): 358-370.